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KMID : 1098420090170010033
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Korean Journal of Medicinal Crop Science
2009 Volume.17 No. 1 p.33 ~ p.38
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Molecular Characterization of the Perilla frutescens Limonene Gene (PFLS) by Agroinfiltration into Nicotiana benthamiana
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Seong Eun-Soo
Seo Eun-Won
Kim Hyoung-Seok
Heo Kweon
Lee Ju-Kyung
Chung Ill-Min
Ghimire Bimal Kumar
Kim Myong-Jo
Lim Jung-Dae
Yu Chang-Yeon
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Abstract
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The full-length cDNA encoding Perilla frutescens limonene synthase (PFLS) (603 amino acids, GenBank accession no. D49368) was cloned. To elucidate the role of PFLS in gene regulation, we transiently transformed full-length PFLS into tobacco plants. PFLS mRNA was first detected in the intact leaves of the plants at 6 h, and the LS transcript level increased after 12 h in leaves treated with oxidative stress-related chemicals. The transient overexpression of PFLS resulted in increased transcription of NbPR1 and NbSIP in Nicotiana benthamiana leaves. Thus, our result confirmed that the infiltration of PFLS gene act as a transcriptional regulator of NbPR1 or NbSIP genes in the tobacco.
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KEYWORD
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NbPR1, NbSIP1, Oxidative Stresses, PFLS, Transient Overexpression
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